Journal: Analytica Chimica Acta

Article Title: A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal amplification without RNA extraction with diagnostic performance same as RT-PCR

doi: 10.1016/j.aca.2022.339590

Figure Lengend Snippet: The impact of mechanical lysis with Zirconium oxide and Chelex on RT-LAMP detection. (A) Effect of vortexing and no vortexing on mechanical lysis of a positive swab sample. (B) Effect of no extraction and subsequent RNA extraction of a mechanically lysed swab sample. (C) RT-PCR detection of extracted RNA from the mechanically lysed sample (D) Mechanical lysis versus other common lysis modalities. (E) RT-PCR detection of extracted RNA from mechanically lysed sample compared with other lysis methods (F) The effect of mechanical lysis time on the release of SARS-CoV-2 RNA. Primer S1:ORF8 (1:0.8) was used in this experiment. Data represent the means ± SD of three technical replicates. The corresponding colorimetric and fluorometric results are shown in Appendix A, Figs. S7 and S8 .

Article Snippet: We detected the purified RNA using an approved commercial real-time RT-PCR kit (Bioperfectus, Jiangsu, China) on a LightCycler® 480 Instrument II (Roche, Mannheim, Germany).

Techniques: Lysis, RNA Extraction, Reverse Transcription Polymerase Chain Reaction

Journal: Analytica Chimica Acta

Article Title: A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal amplification without RNA extraction with diagnostic performance same as RT-PCR

doi: 10.1016/j.aca.2022.339590

Figure Lengend Snippet: Detection of cell-cultured SARS-CoV-2 spiked in 1x phosphate-buffered saline (PBS) and saliva samples in 10-fold dilution series. (PBS, P1-P7) (Saliva, S1-S7). (A, B, C, D) Detection of extracted RNA from PBS and saliva samples. (E, F, G, H) Detection of crude lysates from mechanically lysed PBS and Saliva samples. (A and E, B and F) Colorimetric and fluorometric readouts, respectively. (C, G) Detection of human internal control gene using RPP20 primers. (D, H) Spearman's correlation of RT-PCR with RT-LAMP for PBS and Saliva samples. Primer S1:ORF8 (1:0.8) was used in this experiment.

Article Snippet: We detected the purified RNA using an approved commercial real-time RT-PCR kit (Bioperfectus, Jiangsu, China) on a LightCycler® 480 Instrument II (Roche, Mannheim, Germany).

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: Analytica Chimica Acta

Article Title: A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal amplification without RNA extraction with diagnostic performance same as RT-PCR

doi: 10.1016/j.aca.2022.339590

Figure Lengend Snippet: Diagnostic performance of direct approach RT-LAMP compared to RT-PCR.

Article Snippet: We detected the purified RNA using an approved commercial real-time RT-PCR kit (Bioperfectus, Jiangsu, China) on a LightCycler® 480 Instrument II (Roche, Mannheim, Germany).

Techniques: Diagnostic Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Viruses

Article Title: Epidemiology of Hand, Foot, and Mouth Disease and Genetic Evolutionary Characteristics of Coxsackievirus A10 in Taiyuan City, Shanxi Province from 2016 to 2020

doi: 10.3390/v15030694

Figure Lengend Snippet: Distribution of reported and detected HFMD cases in Taiyuan from 2016 to 2020.

Article Snippet: RNA was detected using three-channel real-time RT-PCR kits (Diagnostic Kit for pan-EV, EV-A71, CVA16, Shanghai ZJ Bio-Tech Co., Ltd., Shanghai, China).

Techniques:

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the invasive ability of HCT-116 cells. (A) Microscopic observation and photography after crystalline violet staining (100× magnification); (B) the OD values of each group at 570 nm wavelength after dissolution of crystalline violet by sodium acetate; (C-G) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction; OD, optical density.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Staining, Dissolution, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the apoptosis of HCT-116 cells. (A,B) Flow cytometric detection of the effects of different transfection groups on apoptosis of HCT-116 cells; (C-I) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and Western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Frontiers in Microbiology

Article Title: Isolation and identification, genome-wide analysis and pathogenicity study of a novel PRRSV-1 in southern China

doi: 10.3389/fmicb.2024.1465449

Figure Lengend Snippet: Identification of porcine reproductive and respiratory syndrome virus (PRRSV) isolates. (A) CPE diagram of PAMs cells infected with PRRSV isolates (left) and PAMS cells blank control (right); (B) CPE diagram of Marc-145 cells infected with PRRSV isolates (left) and Marc-145 blank control (right); (C) Result of indirect immunofluorescence assay of PAMs cells (left) and blank control (right); (D) Result of indirect immunofluorescence assay of Marc-145 cells (left) and blank control (right).

Article Snippet: RT-qPCR amplification was performed using Porcine Reproductive and Respiratory Syndrome Type 1 and Type 2 Multiplex Real-Time PCR Assay Kits (IDEXX, USA) according to the manufacturer’s instructions.

Techniques: Virus, Infection, Control, Immunofluorescence